Restriction Enzymes
New England Biolabs, Inc. BsiWI-HF – 1500 units
A high fidelity restriction endonuclease that recognizes the sequence C^GTACG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. SNAP-Surface Alexa Fluor 488 – 50 nmol
SNAP-Surface Alexa Fluor 488 is a photostable green fluorescent substrate that can be used to label SNAP-tag fusion proteins in solution or on the surface of living cells.
New England Biolabs, Inc. NEBuffer™ 1 – 5 ml
New England Biolabs provides a color-coded 10X NEBuffer (with BSA) with each restriction endonuclease to ensure optimal (100%) activity. This NEBuffer minus BSA formulation is available for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation (which is included with NEB restriction enzymes), select NEBuffer 1.1. New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. The NEBuffer minus BSA formulation is included in this buffer pack for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation, Buffer packs NEBuffer 1.1 (NEB #B7201), NEBuffer 2.1, NEBuffer 3.1 and CutSmart Buffer (NEB #B7204) are available.
New England Biolabs, Inc. BamHI-HF – 50000 units
A high fidelity restriction endonuclease that recognizes the sequence G^GATC_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BtsI-v2 – 500 units
BtsI-v2 is an engineered and improved version of BtsI that recognizes the sequence GCAGTGNN^.
New England Biolabs, Inc. MluI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence A^CGCGT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. EcoRI – 10000 units
A restriction endonuclease that recognizes the sequence G^AATT_C.
New England Biolabs, Inc. ScrFI – 1000 units
A restriction endonuclease that recognizes the sequence CC^N_GG.
New England Biolabs, Inc. BccI – 1000 units
A restriction endonuclease that recognizes the sequence CCATCNNNN^N_.
New England Biolabs, Inc. EcoRI – 10000 units
A restriction endonuclease that recognizes the sequence G^AATT_C.
New England Biolabs, Inc. MspI – 25000 units
A restriction endonuclease that recognizes the sequence C^CG_G.
New England Biolabs, Inc. SfiI – 15000 units
A restriction endonuclease that recognizes the sequence GGCCN_NNN^NGGCC.
New England Biolabs, Inc. NlaIII – 500 units
A restriction endonuclease that recognizes the sequence _CATG^.
New England Biolabs, Inc. BsrBI – 1000 units
A restriction endonuclease that recognizes the sequence CCG_^CTC.
New England Biolabs, Inc. BssSI-v2 – 200 units
BssSI-v2 is an engineered and improved version of BssSI that recognizes the sequence VC^TCGAGB. BssSI-v2 is an engineered and improved version of BssSI. (NEB uses the designation "v2" or "HF" in the name to indicate engineered enzymes.)